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. 2020 Apr 27;10:7034. doi: 10.1038/s41598-020-61803-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of proteins analysed by western blot with corresponding antibodies. (A) Representative western blot images of annexin 1 (ANX1), histone H3 (H3), fibronectin (FN1), high mobility group protein B (HMGB) and dysferlin (DYSF) proteins and (B) quantification of ANX1, H3, FN1, HMGB and DYSF protein expression levels in the Control and Ad-MSCs treated cryopreserved canine semen (Group 1). Data obtained by densitometry showing optical densities relative to the expression in Control. Beta actin expression level served as the loading control. Data are expressed as the mean ± SEM. Different lower case letters a, or b, indicate a significant difference at P < 0.05.