. 2020 Apr 27;10:7034. doi: 10.1038/s41598-020-61803-8

Table 3.

Primer sequences used for gene expression analysis.

Gene	Primer sequence (5′-3′)	Product size (bp)	NCBI accession no.
BACT	F: GAGGCATCCTGACTCTGA	87	XM_544346.3
BACT	R: TCTGGCACCACACTTTCT	87	XM_544346.3
ANX1	F: GAAGCTCTGAAGAAAGCCC	128	NM_001286970.1
ANX1	R: GTGTCTTCATCAGTTCCAAGG	128	NM_001286970.1
H3	F: CGGTGACTGACACGCGAC	136	XM_022404950.1
H3	R: GTTGGAGCAGGCCTTGAACC	136	XM_022404950.1
FN1	F: ATAGCTGGCTGTTACGAC	74	XM_022415242.1
FN1	R: GCATTTCCCAGGTAGGTG	74	XM_022415242.1
HMGB	F: ATATTGCTGCGTACCGAG	64	XM_022409535.1
HMGB	R: TCAGCCTTGACAACTCCC	64	XM_022409535.1
DYSF	F: TGGATCAGAGTGGCGTCC	127	XM_003432223.4
DYSF	R: GACAGCAGCTTTCTGGCT	127	XM_003432223.4

F, forward; R, reverse. Beta actin (BACT), annexin 1 (ANX1), histone H3 (H3), fibronectin 1(FN 1), high mobility group protein B (HMGB), and dysferlin (DYSF).