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. 2020 Apr 27;10:7085. doi: 10.1038/s41598-020-62911-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stimulation with LPS or Mtb lysate induced a glycolytic shift in primary human macrophages. M2 macrophages were stimulated with medium (white circles), Mtb lysate (10 µg/ml; black circles) or LPS (100 ng/ml; grey circles) for 24 h. (A) Macrophage extracellular acidification rate (ECAR) and (B) oxygen-consumption rate (OCR) were measured during sequential injections of D-glucose (10 mM), oligomycin (1 µM) and FCCP (2 µM). (C) Glycolysis (white bars) as determined by the difference in ECAR pre- and post-glucose injection, and OXPHOS (grey bars) as the difference in OCR pre- and post-oligomycin injection. (D) ECAR/OCR ratio. (E) Spare respiratory capacity as determined by the difference in OCR pre-oligomycin and post-FCCP injection. Each symbol represents an individual donor, and bars represent group medians (n = 4). Data is depicted as medians with ranges (n = 4).