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. 2020 Apr 11;17:771–784. doi: 10.1016/j.omtm.2020.04.001

Figure 2.

Figure 2

Effect of the Route of Administration and AAV Serotype on the GFP-Transgene Expression Profile

(A) Spinal cord transduction following LIT-KT injection of the AAVrh10 and AAV9 serotypes or following AAV9 ICV injection. The representative whole spinal cord transversal section indicates rare/no transduction of the ventral horn gray matter for NHPs injected with AAVrh10, whereas transduction is observed primarily in the ventral horn for NHPs injected with LIT-KT and ICV AAV9. Green fluorescence illustrates the native GFP-positive signal and blue the nuclear 4′,6-diamidino-2-phenylindole (DAPI) signal. Hatched windows indicate regions of interest (ROIs) presented in the insets that show higher magnification of the ROI. Main image scale bar, 1 mm; inset scale bar, 100 μm. (B) Representative transduction throughout several brain regions following LIT-KT and ICV AAV9 administration. The green fluorescence signal illustrates the native GFP-positive signal. Scale bars, 150 μm.