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. Author manuscript; available in PMC: 2020 Aug 20.
Published in final edited form as: Biochemistry. 2019 Aug 6;58(33):3480–3493. doi: 10.1021/acs.biochem.9b00355

Figure 1. The structure and sequence of pp32 and its asparagine ladder.

Figure 1.

(A) The hydrogen bond network of the side chain NHz groups of asparagines 74 and 98. Potential hydrogen bonds to backbone carbonyls identified in the crystal structure are shown as dashed lines. (B) Structure of pp32 (PDB ID: 4XOS) with substituted residues shown as sticks. Repeats are colored from blue (N-terminus) to red (C-terminus). Key residues in this study are colored as follows: T49, purple; N74 and N98, gray; L69, green; C123, orange; Y131 and D146, blue. (C) The canonical LRR motif and the pp32 sequence separated into caps and repeats. Residues are aligned based on the HMM logo in Figure S1; minor shifts in the alignment were introduced based on the three-dimensional structure of pp32. Positions corresponding to the LRR motif are highlighted gray.