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. Author manuscript; available in PMC: 2021 Apr 7.
Published in final edited form as: Cell Metab. 2020 Mar 19;31(4):741–754.e5. doi: 10.1016/j.cmet.2020.02.015

Figure 5. Sesn1 regulates cellular and plasma cholesterol content independent of LDLR and SREBP2 signaling.

Figure 5.

(A) Liver PCSK9 and LDLR protein levels in mice after 0.2% cholesterol diet feeding for three days. n = 4.

(B) Plasma total cholesterol levels in Ldlr−/− Sesn1+/+ (black) and Ldlr−/− Sesn1+/− (red) male mice after 4 and 12 weeks of cholesterol diet feeding. n > 9.

(C) Liver mRNA expression levels of cholesterol and lipid genes in male Sesn1+/+ (black) and Sesn1−/− (red) mice fed a cholesterol diet for three days. n = 4.

(D) Cellular cholesterol levels in AML12 cells after Sesn1 silencing (red) or control (black) incubated in FBS or lipoprotein depleted serum (LPDS) medium. n = 4.

(E) LDLR protein levels in Sesn1 siRNA or control siRNA treated AML12 cells with FBS, n = 4.

(F) mRNA expression levels of cholesterol and lipid metabolism genes in AML12 cells after Sesn1 silencing with FBS or LPDS medium. n = 3.

Data represented is mean ± SE, **P < 0.01, *P < 0.05.