Fig. 4.

CAM cultured chick femur defects with Stro-4+ mPCL ECM. Femur with mPCL mesh only (blank scaffold) (A); Femur with mPCL and ECM (B); Femur with mPCL scaffold, ECM hydrogel seeded with Stro-4+ oBMSC (C); N = 3, Scale bar = 2 mm. Micro-Computed Tomography images of day 18 CAM femurs. Stro-4+ mPCL scaffold ECM Hydrogel constructs. Unseeded scaffold alone (Ai & Aii); Unseeded scaffold and blank ECM (Bi & Bii); mPCL scaffold and Stro-4+ Seeded ECM (Ci & Cii). No new bone growth was noted in blank scaffold. Areas of low opacity noted in the blank and blank ECM controls. New bone formation and bony bridging was observed in the Stro-4+ seeded ECM/mPCL scaffold group. N = 3, scale bar = 3 mm.