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. 2020 Apr 27;10:7021. doi: 10.1038/s41598-020-64067-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Senescence (C FDG) (Flow Cytometry) analysis. (A) Example of flow cytometry measurements obtained at D7 for control, 2 and 10 Gy irradiations at 4 MV and 0.63 Gy.min⁻¹. Each bi-parametric representation (Size (FSC)/C FDG (FITC)) represents one independent experiment for at least 5x10 living cells. (B) Each curve (purple and blue for 0.63 and 2.5 Gy.min, respectively) represents the delta of Log FITC compared to control conditions. Each curve represents the mean of three independent experiments based on at least 5x10 living cells for each experiment. (C) The curve represents the delta log FITC (0.63-2.5 Gy.min⁻¹) as a function of the dose. The green arrow represents the range of dose where this delta is significant between the two dose rates (from 2.2 to 20 Gy).