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. 2020 Apr 27;10:7021. doi: 10.1038/s41598-020-64067-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological analysis and lesion scorings after 19 Gy localized intestinal radiation exposure. (A) Representative microscopic alterations obtained in 0.63 and 2.5 Gy.min⁻¹ mice 1 and 6 weeks after irradiation. Slides were stained with hematoxylin-eosin-saffron. Scale bar = 200 μm. (B) A semi-quantitative radiation injury score was assigned to irradiated tissues from both beams 1 and 6 weeks after irradiation (n = 6 animals per group for 1 week end-point, n = 12 animals per group for 6 week end-point, adherences were removed from the figure). (C) Examples of p120 catenin staining performed at 1 week on small intestine section of non-irradiated mice (left panel), and mice irradiated at 2.5 Gy.min⁻¹ (right upper panel) and 0.63 Gy.min⁻¹ (right bottom panel) (original magnification x200). (D) Percentages of bordering epithelium with severe damage (upper panel) and cuboidal cells (bottom panel) (^∗, p < 0.05).