Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 27;11:2031. doi: 10.1038/s41467-020-15834-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Human neutrophils were incubated with C. albicans arrays with Sytox green included in the solution to visualize NET formation during swarming. A cartoon model is shown in (a), demonstrating the distinct phases of swarming and NET release. The area of the neutrophil swarms around C. albicans (b), the mean fluorescent intensity of Sytox green staining in the swarm (c), the number of nuclei in the swarm (d) and the number of nuclei/µm² (e) were all quantified. A red box highlights the timeframe of NET formation and late phase swarm expansion (b–e). N = 68 swarms across three donors for except for the 12 hour timepoint, where N = 60 swarms across three donors (b). N = 44 swarms for (c). N = 12 swarms for d and e. The inclusion of DNase in the media during the swarming assay results in a dose dependent loss of control of C. albicans growth (f). Results are normalized to the growth of the C. albicans alone control. N = 144 swarms over 3 independent donors except for the control PMN group where N = 95 swarms over 3 donors. Error bars represent mean +/− standard deviation.