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. 2020 Apr 27;11:2031. doi: 10.1038/s41467-020-15834-4

Fig. 6. GM-CSF and GCSF enhance neutrophil swarming and fungal restriction.

Fig. 6

GM-CSF and GCSF enhance the restriction of C. albicans without significantly increasing swarm size. The area of swarms with or without GM-CSF or GCSF treatment at 16 hours was quantified and shown. N = 357 swarms for the Control group, N = 331 swarms for the GCSF group and N = 309 for the GM-CSF group, all across 4 independent donors (a). GM-CSF or GCSF treatment further enhanced the delay in C. albicans germination when compared to untreated swarming. N = 40 swarms across 3 donors (b). GM-CSF or GCSF enhanced hyphal restriction, delaying hyphal escape, often for the entire 14 hour assay. N = 40 swarms across 3 donors (c). GM-CSF and GCSF enhanced C. albicans clearance and control. The MFI of C. albicans within swarms at 16 hours was quantified and shown, normalized to the fluorescence of the C. albicans alone condition. N = 285 swarms for the Control group, N = 271 swarms for the GCSF group and N = 284 swarms for the GM-CSF group, all across 4 independent donors (d). The MFI of C. albicans in swarms with or without GM-CSF or GCSF treatment was quantified over 14 hours and is shown. N = 16 swarms from a representative donor (e). n.s. is non-significant, **p ≤0.01 (p= 0.003 control vs GCSF, p < 0.0001 for control vs GM-CSF) and ****p <0.0001 (c, d). Kruskal-Wallis with Dunns post-test. Error bars represent mean +/− standard deviation for (a–d) and mean +/− standard error for (e).