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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Exp Hematol. 2019 May 25;74:52–63.e3. doi: 10.1016/j.exphem.2019.05.002

Figure 1. Healthy donor derived CD123 CAR T cells target and eliminate CD123+ MDS-L cell line.

Figure 1.

(A) MDS-L cells were cocultured for 48hrs at E:T ratio of 1:1 with mock T cells, CD19 CAR T cells, or CD123 CAR T cells, stained for CD3 and CD123 and analyzed by flow cytometry (mean ± SD of duplicates, n = 2 healthy donors with 3–5 technical duplicates). (B) Statistical analyses of flow cytometry showing elimination of CD123+ MDS-L cells by CD123 CAR T cells (red), but not mock T cells (blue), or CD19 CAR T cells (green). (C) Mock T cells (blue), CD19 CAR T cells (green), CD123 CAR T cells (red) were culture with and without MDS-L cells for 5hrs at E:T ration of 1:1. Presence of CD123 antigen on MDS-L cells leads to increase of CD107A+ and TNF-α+ in CD123 CAR T cells, but not in mock T cells and CD19 CAR T cells (mean ± SD of duplicates, unpaired parametric t-test. NS: p > 0.05, * p ≤ 0.05, ** p ≤ 0.01).