FIGURE 1.

SADS‐CoV PLP2 cleaves nsp1‐eGFP. Time‐dependent cleavage of nsp1‐eGFP by SADS‐CoV PLP2. A 200 nM of nsp1‐eGFP was incubated with 20 nM of PLP2 at 23°C for the incubated time and then the mixture was analyzed by SDS‐PAGE. The protease activity was indicated by the release of free nsp1 and eGFP