Figure 1.

BioID2‐p38α fusion protein for proximity labeling in cultured cells. (a) Schematic of expression constructs for myc‐BioID2 control and myc‐BioID2‐p38α. (b) Immunoblots of 293T cells expressing either BioID2 (n = 2), BioID2‐p38α (n = 2), or enhanced green fluorescence protein (eGFP) (n = 1) and incubated with biotin (0.2 mM) for 24 hr. Protein expression was detected by immunoblot probed for myc tag or β‐actin as loading control. Biotinylated polypeptides were detected by probing with streptavidin–horseradish peroxidase (HRP) conjugate