Figure 6.
Proximity ligation assay (PLA) confirms interaction of p38α with XPA and proliferating cell nuclear antigen (PCNA) upon UV irradiation. (a) PLA signals for combination of anti‐HA and anti‐p38α in 293T cells expressing FLAG‐p38α or with empty vector (EV) transfection. Cells were UV irradiated (350 J/m2) or mock treated as indicated. DAPI, nuclei. Scale bar, 5 μm. (b) Quantification of HA‐p38α PLA signal in cells expressing FLAG‐p38α and HA‐XPA with UV or mock treatment (n = 64–211 cells per condition). * p < .05 (Student's t test). (c) PLA signals for combination of anti‐PCNA and anti‐p38α in 293T cells expressing FLAG‐p38α and/or HA‐XPA. EC control, EV control. Cells were UV irradiated (350 J/m2) or mock treated as indicated. DAPI, nuclei. Scale bar, 5 μm. (d) Quantification of PCNA‐p38α PLA signal in cells expressing FLAG‐p38α with UV or mock treatment (n = 29–35 cells per condition). * p < .05 (Student's t test)