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. 2019 Dec 13;221(10):1636–1646. doi: 10.1093/infdis/jiz663

Figure 1.

Figure 1.

Mycobacterium tuberculosis (Mtb) HN878 infection induces human-like TB granulomas in lungs of mice. (A and B) C57BL/6 mice were aerosol infected with ~100 colony-forming units of Mtb H37Rv (Rv) or Mtb HN878 (HN) and sacrificed at 50, 100, and 150 days postinfection (dpi). Formalin-fixed and paraffin-embedded (FFPE) lung sections were stained with hematoxylin and eosin, and samples were histologically assessed. (A) The Rv infection is representative at 50 dpi. (B) The HN infection is representative of 50 dpi. (C) Human biopsy specimen obtained from pulmonary tuberculosis (TB) patient (left panel) and nonhuman primates with pulmonary TB that were infected with Mtb Erdman (right panel, 13 weeks postinfection). (D) The number of human-like granulomas per mouse lung lobe was determined histologically in FFPE lung sections. Area occupied by human-like granulomas quantified using the morphometric tool of the Zeiss Axioplan microscope. Percentage from lung area occupied by human-like granulomas per mouse lung lobe was determined. The data shown represent mean (±standard deviation) values from 2 to 3 independent experiments per time point (n = 4–5 mice per group in each experiment). Student t test was used to determine differences per time point. *, P < .05.