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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Mol Immunol. 2020 Feb 26;120:93–100. doi: 10.1016/j.molimm.2020.01.018

Fig. 3. M3814 Inhibits DNA-PKcs Kinase Activity.

Fig. 3.

A p53 peptide was used to measure DNA-PKcs kinase activity in the presence of M3814. DNA-PKcs (20 nM) was incubated with p53 peptide (250 μM), 1 μM dsDNA, and 80 nM [γ−32P] ATP at37 °C for 10 min. in 25 mM Tris−HCl (pH 7.5), 10 mM MgCl2,10 mM DTT, and 5 % sucrose. Inhibition was measured by comparing the amount of P-p53 in each sample relative to the DMSO control (Lane 3).