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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Mol Immunol. 2020 Feb 26;120:93–100. doi: 10.1016/j.molimm.2020.01.018

Fig. 4. M3814 Inhibits Artemis:DNA-PKcs Complex.

Fig. 4.

A fluorescently-labeled substrate (500 nM) was used to determine whether M3814 can inhibit Artemis (10 nM) by inhibiting DNA-PKcs (10 nM) (4). Reactions were performed in 25 mM Tris−HCl, pH 8.0, 10 mM KCl, 10 mM MgCl2, and 1 mM DTT incubated for 30 min at 37 °C. DNA-PKcs was treated with M3814, and inhibition was measured by comparing substrate-to-product conversion relative to the DMSO control (Lane 3). A visual description of substrates and products is provided on the right-hand side of the figure, and the sequences are in the Methods.