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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Mol Immunol. 2020 Feb 26;120:93–100. doi: 10.1016/j.molimm.2020.01.018

Table 1. Inhibits V(D)J Recombination in 697 Cells.

Artemis: DNA-PKcs is required to open double-stranded DNA hairpins generated during V(D)J recombination. Inhibiting DNA-PKcs would prevent this action and result in a decrease in CJ formation and perhaps SJ formation in V(D)J recombination. This assay measures % replication and % recombination in 697 cells upon treatment with 0.5 μM, 1 μM, and 1.5 μM M3814. Values marked with an asterisk (*) are significant compared to the DMSO control (p < 0.05).

Signal Joint Coding Joint
M3814 % replication % recombination % replication % recombination
0.5 μM − 4.0 7.0 2.8 1.02
+ 5.6 3.2 6.7 0.18*
1 μM − 6.2 7.9 10.3 1.5
+ 7.4 1.1* 10.1 < 0.004*
1.5 μM − 16.7 4.0 26.1 0.6
+ 7.7 0.34* 8.1* 0.003*