Table 2. Does Not Affect Junction Fidelity.
Recombinant plasmids containing signal or coding joints were sequenced to determine the fidelity of junction formation. Tables 2a and 2b show sequences collected from the 0.5 μM and 1.5 μM V(D)J recombination data sets, respectively.
| a. 0.5 micromolar M3814 V(D)J Recombination | |||
|---|---|---|---|
| Signal Junctions | |||
| Sample | CACTGTG | Junctional Additions | CACAGTG |
| 1 | CACTGTG | CACAGTG | |
| 2 | CACTGTG | CACAGTG | |
| 3 | CACTGTG | CACAGTG | |
| 4 | CACTGTG | CACAGTG | |
| 5 | CACTGTG | TCCCG | CACAGTG |
| 6 | CACTGTG | GG | CACAGTG |
| 7 | CACTGTG | TGGCTT | CACAGTG |
| Coding Junctions | |||
| Sample | TGCAGGTCGAC | Junctional Additions | GGATCCCCGGG |
| 1 | TGCAGGTC— | -GATCCCCGGG | |
| 2 | TGCAGGTC— | C | -GATCCCCGGG |
| 3 | TGCAGG—— | CC | -GATCCCCGGG |
| 4 | TGCAGGTCG– | CC | GGATCCCCGGG |
| 5 | TGCAGGTCG– | CC | GGATCCCCGGG |
| b. 1.5 micromolar M3814 V(D)J Recombination | |||
| Signal Junctions | |||
| Sample | CACTGTG | Junctional Additions | CACAGTG |
| 1 | CACTGTG | CACAGTG | |
| 2 | CACTGTG | GG | CACAGTG |
| 3 | CACTGTG | CC | CACAGTG |
| 4 | CACTGTG | GCC | CACAGTG |
| 5 | CACTGTG | CACAGTG | |
| 6 | CACTGTG | CACAGTG | |
| 7 | CACTGTG | G | CACAGTG |
| Coding Junctions | |||
| Sample | TGCAGGTCGAC | Junctional Additions | GGATCCCCGGG |
| 1 | TGCAGGTC— | -GATCCCCGGG | |
| 2 | TGCAGGTCGAC | ——CCCCGGG | |
| 3 | TGCAGGTCGAC | ——CCCCGGG | |
| 4 | TGCAGGTCGA- | —TCCCCGGG | |
| 5 | TGCAGGTCGAC | ——CCCCGGG | |
| 6 | TGCAGGTCGAC | CACTGTG | GGATCCCCGGG |
| 7 | TGCAGGTCGA- | —TCCCCGAG | |
| 8 | TGCAGGTCGAC | ——CCCCGTG | |
| 9 | TGCAGGTCGAC | GGATCCCCGGG | |
| 10 | TGCAGGTCGA- | —TCCCCGGG | |
| 11 | TGCAGGTCGAC | GGATCCCCGGG | |
| 12 | TGCAGGTC— | –ATCCCCGGG | |
| 13 | TGCAGGTC— | -GATCCCCGGG | |
| 14 | TGCAGGT—— | -GATCCCCGGG | |
| 15 | TGCAG——— | C | -GATCCCCGGG |
| 16 | TGCAGGTCGAC | ——CCCCGGG | |
| 17 | TGCAG———— | CCCT | GGATCCCCGGG |
| 18 | TGCAGGTCGAC | ——CCCCGGG | |