Fig. 6.

(A to F). Least square means (LSM) and standard error of the mean (SEM) for percentage of CD4⁺ (A), CD8⁺ (B), WC1⁺ (C), CD4⁺CD25⁺ (D), CD8⁺CD25⁺ (E), and WC1⁺CD25⁺ (F) T-cell phenotype in peripheral blood circulation of beef calves treated (VAC + ITM) or not (VAC + SAL) with injectable trace minerals (ITM) concurrently with MLV-BRD vaccination, or calves not vaccinated or treated (UNVAC). Calves received vaccination and treatment (ITM or saline) on study day -5, and were individually challenged with ncp BVDV2 (strain 890) five days later (day 0). Errors bars represent the SEM. ^{x, y} Significant difference between groups on each study day (P < 0.05). ^† Significant difference relative to day 0 for VAC + ITM (P < 0.05). ^‡ Significant difference relative to day 0 for the VAC + SAL group (P < 0.05). ^¶ Significant difference relative to day 0 for UNVAC (P < 0.05).