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. Author manuscript; available in PMC: 2020 Apr 27.
Published in final edited form as: Bone. 2020 Feb 4;133:115262. doi: 10.1016/j.bone.2020.115262

Figure 3: ectonucleotidase activity of TNAP in hypertrophic chondrocytes and osteoblasts.

Figure 3:

Mouse primary chondrocytes (A, C and E) and primary osteoblasts (B, D and F) were cultured as detailed in the Materials and methods section for 16 days. Hydrolysis of the substrate (10 μM ATP or 25 μM AMP) into Pi was measured by the Malachite green assay in the presence or absence of 25 μM of MLS-0038949 and 100 μM of ARL-67156 (A, n=5 and B, n=4), or in the presence or absence of 25 μM of MLS-0038949 and 100 μM of AOPCP (E, n=5 and F, n=4). Endogenously released ATP was quantified with the bioluminescent Luciferin-Luciferase reaction in presence (ο) or absence (□) of MLS-0038949 (C, n=5 and D, n=3). * indicates a statistical difference with p<0.05; ** a difference with p<0.01; *** a difference with p<0.001.