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. Author manuscript; available in PMC: 2020 Apr 27.
Published in final edited form as: Bone. 2020 Feb 4;133:115262. doi: 10.1016/j.bone.2020.115262

Figure 6: anti-inflammatory ectonucleotidase function of TNAP in neutrophils.

Figure 6:

A: TNAP activity measured in freshly isolated human neutrophils (n=5 for neutrophils and n=3 for PBMCs). B: effect of 0.5 μg/mL of LPS on ALPL mRNA levels as determined by RT-qPCR (n=6 independent experiments from 4 different donors). C: RT-qPCR analysis of nucleotidase transcripts in freshly isolated human neutrophils (n=5; nd: not detected). D: quantification by ELISA of extracellular levels of IL-1β in neutrophils primed with 0.5 μg/mL of LPS for 3 h and further stimulated with 2 mM of ATP during 45 min (n=9 independent experiments from 4 different donors). A representative western-blot experiment of intracellular IL-1β is shown. E: quantification of cell viability and cell death with MTT and LDH assays respectively in cells treated with 25 μM of MLS-0038949 for 24 h (n=7 independent experiments from 4 different donors). * indicates a statistical difference with p<0.05; ** a difference with p<0.01; *** a difference with p<0.001.