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. Author manuscript; available in PMC: 2020 Apr 27.
Published in final edited form as: Bone. 2020 Feb 4;133:115262. doi: 10.1016/j.bone.2020.115262

Table 1: Summary of primers used.

Shown are the mouse (m) and human (h) primer sequences (F: forward; R: reverse), annealing temperatures (Ta), base pair (bp) lengths of the corresponding PCR products, and GenBank accession numbers.

Gene GenBank Ta (°C) Sequence Length (bp)
mGapdh NM_008084.3 60 F: 5’- GGCATTGCTCTCAATGACAA-3’ 200
R: 5’-TGTGAGGGAGATGCTCAGTG-3’
mAlpl NM_007431.3 60 F: 5’-CAAAGGCTTCTTCTTGCTGGT-3’ 258
R: 5’-AAGGGCTTCTTGTCCGTGTC-3’
mBglap NM_001037939.2 60 F: 5’-AAGCAGGAGGGCAATAAGGT-3’ 364
R: 5’-CGTTTGTAGGCGGTCTTCA-3’
mCol10a1 NM_009925.4 60 F: 5’-CAAACGGCCTCTACTCCTCTGA-3’ 129
R: 5’-CGATGGAATTGGGTGGAAAG-3’
mIl-10 NM_010548.2 60 F: 5’-CCCTTTGCTATGGTGTCCTTTC-3’ 100
R: 5’-GATCTCCCTGGTTTCTCTTCCC-3’
mIl-1β NM_008361.4 62 F: 5’-GGGCCTCAAAGGAAAGAATC-3’ 152
R: 5’-CCACTTTGCTCTTGACTTCTATC-3’
mIl-6 NM_031168 60 F: 5′-GTCACAGAAGGAGTGGCTA-3′ 193
R: 5’-AGAGAACAACATAAGTCAGATACC-3’
mIL1-ra NM_031167 60 F: 5′-GGGATACTAACCAGAAGACC-3’ 157
R: 5’-GACAGGCACAGCTTGCCCCC-3’
mTnf-α NM-013693 60 F: 5′-TGGGACAGTGACCTGGACTGT-3’ 67
R: 5’-TTCGGAAAGCCCATTTGAGT-3’
mNpp1 NM_001308327.1 60 F: 5′-CGGACGCTATGATTCCTTAGA-3′ 93
R: 5’-AGCACAATGAAGAAGTGAGTCG-3’
mCd39 NM_001304721.1 60 F: 5’-AGGTGAAGAGATTTTGCTCCAA-3’ 101
R: 5’-TTTGTTCTGGGTCAGTCCCAC-3’
mCd73 NM_011851.4 60 F: 5’-GGACATTTGACCTCGTCCAAT-3’ 191
R: 5’-GGGCACTCGACACTTGGTG-3’
hRPLP0 NM_001002.3 60 F: 5’-CGACCTGGAAGTCCAACTAC-3’ 289
R: 5’-AGCAACATGTCCCTGATCTC-3’
hGAPDH NM_002046.5 60 F: 5’-GTTCCAATATGATTCCACCC-3’ 487
R: 5’-AGGGATGATGTTCTGGAGAG-3’
hALPL NM_000478.4 60 F: 5’-CAAAGGCTTCTTCTTGCTGGT-3’ 258
R: 5’-AAGGGCTTCTTGTCTGTGTC-3’
hNPP1 NM_006208.2 60 F: 5’-CGATTTTGCCGATTGAGGATT-3’ 146
R: 5’-AAACTGGTGCTGGGAAAGAAGACA-3’
hCD39 NM_001776.5 60 F: 5’-CTGATTCCTGGGAGCACATC-3’ 99
R: 5’-CTGGGATCATGTTGGTCAGG-3’
hCD73 NM_002526.3 60 F: 5’-CTCCTCTCAATCATGCCGCT-3’ 221
R: 5’-CAAATGTGCCTCCAAAGGGC-3’