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. Author manuscript; available in PMC: 2020 Oct 15.
Published in final edited form as: Nature. 2020 Apr 15;580(7804):511–516. doi: 10.1038/s41586-020-2199-7

Extended Data Figure 9: Genetic silencing of GPR65 neurons does not affect the development of sugar preference.

Extended Data Figure 9:

a, Global silencing of the GPR65 neurons was achieved by generating GPR65-IRES-Cre; R26-TeNT double transgenic animals expressing TetTox in GPR65 neurons. Sugar-preference graphs demonstrating the robust development of preference for sugar versus artificial sweetener for both Wild Type (n = 5 mice, two-tailed paired t-test, p = 0.0047) and GPR65:TetTox mice (n = 5 mice, two-tailed paired t-test, p = 0.0033). The Wild Type controls shown here are the same animals used in Extended Data Fig. 3c as both sets of silencing experiments were carried out as part of the same series of studies. Values are mean ± s.e.m. b, Silencing of GPR65 neurons does not impair the innate attraction to sweet solutions. Shown are behavioural responses to AceK versus water and glucose versus water (n = 5, two-tailed paired t-test, p = 0.0040 for consumed volumes of AceK vs water, p = 0.0023 for consumed volumes of glucose vs water). Values are mean ± s.e.m.