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. Author manuscript; available in PMC: 2021 Apr 24.
Published in final edited form as: Circ Res. 2020 Apr 23;126(9):1112–1126. doi: 10.1161/CIRCRESAHA.119.315940

Figure 1.

Figure 1.

Experimental workflow for CITE-seq and AB-seq experiments.

Single cell suspensions derived from artery wall (top) or PBMCs (bottom) are sorted for live cells and other desired markers (here: CD45 for leukocytes), hash-tagged, washed and incubated with the oligonucleotide-marked antibody panel (currently 50 mAbs are possible). After 3 more washes, the cells are loaded into the BD Rhapsody scanner or the 10x Chromium controller. Beads are retrieved and processed for library preparation. Pre-sequencing quality control (QC) is done by TapeStation. DNA fragments are removed, and the library is sequenced using NGS sequencers like the Illumina NovaSeq. The figure was created with BioRender.