Figure 2. WFA synergizes the antiproliferative activity of PAC and CisPt against H1299 cells.

The potency and efficacy of PAC, CisPt, and WFA against H1299 cells were compared using the median-effect equation. (A) Dose-response plots depicting PAC, CisPt and WFA dose-dependent inhibition of cell proliferation of H1299 cells. (B) Median-effect plot indicated PAC had lowest IC₅₀ followed by WFA and CisPt the highest. Preliminary combinations of PAC and CisPt, PAC and WFA and CisPt and WFA were tested. PAC+WFA (C) displayed the greatest efficacy, followed by CisPt + WFA (D–F). Isobologram analysis (G–I) and combination index (CI) values (<1) indicated that all the combinations were highly synergistic against H1299 cells (J–L).