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. 2020 Apr 21;11(16):1399–1416. doi: 10.18632/oncotarget.27519

Figure 5. The combination of PAC and WFA synergistically induced apoptosis and inhibited colony formation in NSCLC cells.

Figure 5

Apoptosis detection was performed by (A) Annexin-V/PI assay and (B) Western blot analysis. The combination of PAC and WFA synergistically increased the percentage of annexin-V positive cells as compared to each drug alone. There was an increase in the cleavage of PARP, caspase 3, increased Bax but decreased the expression of Bcl2. Representative images showing the colony formation assay in H1299 (C) and A549 (D) cells incubated with PAC and WFA alone and in combination. In both cell lines, PAC and WFA alone dose-dependently inhibited colony formation.