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. Author manuscript; available in PMC: 2020 Sep 30.
Published in final edited form as: Nat Cell Biol. 2020 Mar 30;22(4):372–379. doi: 10.1038/s41556-020-0493-0

Figure 2. The progesterone receptor interacts with RNA-helicase DDX21.

Figure 2.

(a, b) In situ hybridization at 24hpf for crestin in control, pgr morpholine (MO) and pgr mRNA injected embryos exposed to leflunomide in the presence and absence of progesterone. Number of embryos displaying the presented phenotype is indicated. Scale bars represent 200μm. (c) qPCR for pgr on zebrafish embryos at 24hpf using two different primer sets. (pgr primer set 1; n = 4, pgr primer set 2; n = 3 biologically independent experiments, Mean ± SD, Unpaired T-test with Welch Correction, * p = 0.017, ** p = 0.002, *** p = 0.021). (d) Co-Immunoprecipitation followed by mass-spectrometry of pgr:flag:T2A:gfp injected embryos identified complex association partners of Pgr in vivo. (e) DDX21 associates with PGR in A375 melanoma cells (2 biologically independent experiments). Source data are available online.