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. 2020 Apr 8;12(7):6129–6150. doi: 10.18632/aging.103013

Figure 3.

Figure 3

FOXK1 inhibition inhibits the invasion and metastasis of GC cells in an acidic microenvironment in vitro. (A) Wound healing assays were performed to detect changes in the migration of MGC803 and AGS cells at pH 6.5. The analysis of wound healing was performed using ImageJ software. Scale bar, 500 μm. (B) Pretreatment of MGC803 and AGS cells at pH 6.5. Transwell assays were performed to assess the migration capacity of GC cell lines. Scale bar, 500 μm. (C) Transwell assays were performed to assess the invasive ability of acidic MGC803 and AGS cells. Scale bar, 500 μm. The data are presented as the means ± S.D.s from three independent experiments. * P < 0.05, **P < 0.01 and *** P < 0.001.