Figure 3.

Minor impacts of RMF on generation of Th1/Th2/Th17 cytokines as well as T cell propagation. Cytokines in serum from the treated EAE mice were detected at (A) day 10 and (B) day 20 after immunization. (C) Spleen cells and (D) lymph node cells derived from the treated EAE mice at day 10 following vaccination were re-activated with 10 μg/mL of MOG35–55 peptide. (C, D) Propagation was detected after 72 h of incubation. (E) IFN-γ, IL-2, IL-4 as well as IL-17A were detected following 48 h of incubation. Data are shown as the mean ± SEM of three independent experiments. *P <0.05.