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. 2020 Apr 1;12(7):5693–5715. doi: 10.18632/aging.102958

Figure 2.

Figure 2

IHH knockdown induced BMSC senescence. (A) BMSC transfected with NC siRNA and IHH siRNA stained by SA-β-gal stain. (B) BMSC (n = 5) were transfected with siRNA negative control or siRNA IHH for 24hours. TP53, CDKN2A, SA-β-gal, mTOR, PIK3, GDF11, COX-2, IDO1, IL-6, HGF, and TGFβ genes expressions were measured by RT-PCR. GAPDH was used as a housekeeping gene. (C) BMSC (n = 5) were transfected with siRNA negative control or siRNA IHH for 48hours. P53, P16, PI3K, and p-PI3K proteins expressions were measured by Western Blot. β-actin was used as an internal control. Results presented as mean ± SEM. *P <0.05, **P < 0.01, ***P < 0.001, ****P.