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IHH shortage-induced aging-associated genes suppressed by mTOR and ROS inhibition in BMSC. siRNA IHH-transfected BMSC (n=5) were incubated with or without INK128, DPI, or NAC for 24hours. TP53 (A), CDKN2A (B), SA-β-gal (C), PIK3 (D) and GDF11 (E) genes expressions were measured by RT-PCR. GAPDH was used as a housekeeping gene. All results were normally distributed and shown as mean ± SEM. *P <0.05, **P < 0.01, ***p <0.001, ****p <0.001.
