Table 2. Primers sequences used in gene amplification.
| Gene | Forward Primer | Reverse Primer |
| GAPDH (272 bp) | TGACCACAGTCCATGCCATCAC | CGCCTGCTTCACCACCTTCTT |
| IHH(247 bp) | GAACTCGCTGGCTATCTCGG | CTCGGACTTGACGGAGCAAT |
| PTCH1(298 bp) | TGTCGCACAGAACTCCACTC | ACCAAGAGCGAGAAATGGCA |
| PTCH2(431 bp) | TTACCGCAACTGGCTACAGG | CGATGGCCTCCACAAAGTCT |
| P53 (463 bp) | GCTTGCAATAGGTGTGCGTC | AAACTACCAACCCACCGACC |
| CDKN2A (221 bp) | CCACCCCGCTTTCGTAGTT | CCACATGAATGTGCGCTTAGG |
| PPARγ (513bp) | TTTGGGATCAGCTCCGTGG | CATCCGCCCAAACCTGATG |
| mTOR (541) | CTTAGAGGACAGCGGGGAAG | TCAGCGGTAAAAGTGTCCCC |
| PIK3 (207 bp) | ACTCACCTTCTGCTCCGTTG | TCATACTCGCGGCTCTTGTC |
| GDF11 (546 bp) | GACCAAGCCGTGTGCAATAC | AAGGGATAAACGGGGCACAG |
| COX-2 (146 bp) | TGACCACAGGCAGATGAA | CCACAGCATCGATGTCACCATAG |
| IDO1 (159 bp) | GAATGGCACACGCTATGGAA | CAGACTCTATGAGATCAGGCAGATG |
| IL-6 (234bp) | CCTTCGGTCCAGTTGCCTTCTC | CCAGTGCCTCTTTGCTGCTTTC |
| HGF (147 bp) | GTCAGCCCTGGAGTTCCATGATA | AGCGTACCTCTGGATTGCTTGTG |
| TGFβ (130 bp) | AGCGACTCGCCAGAGTGGTTA | GCAGTGTGTTATCCCTGCTGTCA |
| SA-β- gal (268 bp) | CGACTATGATGCCCCACTGA | TGTCCGGTACAGCACAAACC |
| FABP4 (149 bp) | ATGGGGGTGTCCTGGTACAT | ACGTCCCTTGGCTTATGCTC |
| LPL (473 bp) | AGTAGCAGAGTCCGTGGCTA | GGGACCCTCTGGTGAATGTG |
| ALP-BMA (339 bp) | CGGAAGACACTCTGACCGTG | GGACGTAGTTCTGCTCGTGG |
| RUNX2 (434 bp) | CAGTGGCCCAGTGGTATCTG | GAGGGCACTGGTCCATACAC |
| Osteocalcin (277 bp) | ACCATGAGAGCCCTCACACTC | CCTCCTGAAAGCCGATGTGG |