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. 2020 Apr 13;12(7):6058–6066. doi: 10.18632/aging.103002

Figure 3.

Figure 3

Aspirin inhibited the activation and proliferation of hepatic stellate cells. (A, B) Real-time PCR and western blot was employed to examine the expression of α-SMA, collagen-a1, TGF-β, IL-6 and TNF-α in the LPS-treated HSCs with disposure of 0, 10, 20, 40mmol/L. (C, D) ELISA assay was used to examine the expression of TGF-β, IL-6 and TNF-α in the LPS-treated HSCs with disposure of 0, 10, 20, 40mmol/L. (E, F) Real-time PCR and western blot was employed to examine the expression of α-SMA, collagen-a1, TGF-β, IL-6 and TNF-α in the LPS-treated HSCs with disposure of 40mmol/L at 48 and 72h. (G, H) ELISA assay was used to examine the expression of TGF-β, IL-6 and TNF-α in the LPS-treated HSCs with disposure of 40mmol/L at 48 and 72h. (I) CCK-8 assays were performed to examine the proliferation of LPS activated-HSCs with aspirin treatment. (J) LDH assay was performed to detect the cell viability of LPS activated-HSCs with aspirin treatment. (K, L) The expression of Cyclin D1 was detected by real-time PCR and western blot. *P<0.05, **P<0.01, ***P<0.001.