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. 2020 Apr 8;12(7):6172–6190. doi: 10.18632/aging.103015

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Copyright © 2020 Wei et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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BCAT1 was targeted by miR-934 in OS cells. (A) Heatmap of differentially expressed genes in GSE36001. (B) Volcano map. (C) Venn diagram showed the intersection of up-regulated genes in GSE36001 dataset, “miRDB” predicted miR-934 target genes and “starbase” predicted miR-934 target genes. (D) Relative BCAT1 expression in GSE36001 dataset. (E) Relative BCAT1 expression in 157 OS samples. (F) qPCR analyses detected the relative BCAT1 levels. (G) CCK-8 assays. (H) Clonogenic assays. (I) Binding sites between miR-934 and 3’UTR of BCAT1 mRNA. (J) Luciferase activity detection. (K) Real-time PCR analyses measured relative BCAT1 levels. * P < 0.05, **P < 0.01.