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. 2020 Apr 3;19(6):3581–3588. doi: 10.3892/etm.2020.8644

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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miR-663b inhibitor increases hypoxia-induced H9c2 cell viability and reduces apoptosis. (A) An MTT assay was used to detect cell viability. (B) Flow cytometry combined with (C) annexin V/PI staining was performed to measure apoptosis. (D) Western blotting was used to demonstrate cleaved-Caspase-3 protein expression levels in H9c2 cells. Data are presented as the mean ± standard deviation of three independent experiments. ^**P<0.01 vs. control group; ^##P<0.01 vs. hypoxia group; ^&&P<0.01 vs. hypoxia + inhibitor group. PI, propidium iodide; miR, microRNA; si, small interfering.

miR-663b inhibitor increases hypoxia-induced H9c2 cell viability and reduces apoptosis. (A) An MTT assay was used to detect cell viability. (B) Flow cytometry combined with (C) annexin V/PI staining was performed to measure apoptosis. (D) Western blotting was used to demonstrate cleaved-Caspase-3 protein expression levels in H9c2 cells. Data are presented as the mean ± standard deviation of three independent experiments. ^**P<0.01 vs. control group; ^##P<0.01 vs. hypoxia group; ^&&P<0.01 vs. hypoxia + inhibitor group. PI, propidium iodide; miR, microRNA; si, small interfering.