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. Author manuscript; available in PMC: 2020 Apr 27.
Published in final edited form as: Hum Mutat. 2020 Jan 16;41(4):786–799. doi: 10.1002/humu.23969

FIGURE 4.

FIGURE 4

Neuro-organoid failed to form in siRad9B treated culture. (a) Culture scheme for establishing the neural-organoid (“mini-brain”). (b) Brightfield imaging landscape (×4; scale bar = 1 mm, ×10; scale bar = 400 μm) of the control and siRad9B treated neural-organoid culture. (c) Immunostaining on the neuro-organoid structure. Scale bar = 50 μm. Two parallel experiments are performed; each experiment has triplicate wells for staining