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. 2019 Oct 21;32(1):57–68. doi: 10.1093/intimm/dxz060

Fig. 5.

Fig. 5.

MMP inhibition restores the capacity of CD-MFs to suppress activated Th1/Th17 cell responses. MFs were co-cultured with CD3/CD28 pre-activated allogeneic CD4+ T cells for 5 days in the presence or absence of the MMP inhibitor II (30 nM) or anti-MMP-10 (1 μg ml−1) blocking mAbs. (a) Tbet and IFN-γ mRNA expression by the T cells was analyzed using real-time RT–PCR analysis. (b) RORC and IL-17A mRNA expression by the T cells was analyzed using real-time RT–PCR analysis. (c) IFN-γ and IL-17A production was analyzed using multiplex cytokine analysis. The means ± SEM are shown, n = 4 allogeneic donor pairs per group, two experimental replicates each, *P < 0.05; **P < 0.01.