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. 2020 Apr 16;9(1):1747350. doi: 10.1080/2162402X.2020.1747350

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© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Regulation of the tumor microenvironment by vaccines. (a, b, c) Representative flow cytometry plots (left) and quantification (right) of frequencies of intratumoral FAPα⁺ CAFs (a), CD3⁺CD8⁺ T cells (b), and CD11b⁺Gr-1⁺ MDSCs (c). (d, e) Relative mRNA expression levels of survivin (BIRC5), FAPα, collagen I (COL1A1), CXCL12, CCL2 (d), and IL-2, TNF-α, IFN-γ, IL-6, IL-10, GM-CSF (CSF2), and G-CSF (CSF3) (e) in tumors isolated from vaccinated tumor-bearing mice were detected by qRT-PCR. Gapdh was used as an endogenous control. (*P < .05, **P < .01, ***P < .001, ****P < .0001).