Figure 4.

Regulation of estrogen receptor β-mediated PI3K/Akt signaling in osteosarcoma MG-63 cells by calycosin. (A) Western blotting results of (B) protein expression levels of p-PI3K/PIK3 and p-Akt/Akt after treatment with different concentrations of calycosin. (C) Western blotting results of (D) protein expression levels of p-PI3K/PIK3 and p-Akt/Akt after treatment with 100 µM calycosin, in the presence or absence of PHTPP for 48 h. These independent experiments were performed three times. *P<0.05, **P<0.01 vs. 0 µM calycosin. ^##P<0.01 vs. calycosin. ^&P<0.05 vs. PHTPP. p, phosphorylated.