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. 2020 Mar 30;21(6):2493–2501. doi: 10.3892/mmr.2020.11045

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — miR-378 regulates genes related to apoptosis and proliferation of HuH-7 cells more efficiently. (A) Western blot images showing protein expression of Cyclin D1, Bcl-2, Bax, Akt, p53 and Survivin in Blank, miR-378 control, L and LUS groups. Histograms showing (B) relative protein and (C) mRNA expression levels in each group. (D) Western blotting was used to measure the expression of β-catenin. (E) Relative expression of β-catenin was quantified via western blotting. Bars indicate mean ± SD. *P<0.05, **P<0.01 and ***P<0.001 vs. Blank; ^{^^}P<0.01 and ^{^^^}P<0.001 vs. miR-378 control; ^#P<0.05, ^##P<0.01 and ^###P<0.001 vs. L. L group, the hepatocellular carcinoma cells were transfected with miR-378; LUS group, the hepatocellular carcinoma cells were treated with miR-378 mimic combined with Ultrasonic irradiation and SonoVue microbubbles. miR, microRNA.