Figure 2.

Suppression of CD44 may decrease the formation of type II collagen and aggrecan. (A) Expression of type II collagen in NC and PG groups in P3 hAMSCs on day 7 of treatment with 2 µg/ml A3D8 by immunocytochemistry staining. The red circle indicates type II collagen; the nuclei were counterstained with hematoxylin (blue). (B) Toluidine blue staining for formation of aggrecan in NC and PG groups on day 7 of treatment with 2 µg/ml A3D8. Blue staining denotes aggrecan. Scale bar, 200 µm. NC, negative control group; PG, positive drug group; hAMSCs, human amniotic mesenchymal stem cells.