Figure 4.

Suppression of CD44 inhibits ERK1/2 signaling during the chondrogenic differentiation of hAMSCs. Change in transcriptional levels of (A) ERK1 and (B) ERK2 in P3 hAMSCs after 7 days of inhibition of CD44 with 2 µg/ml A3D8. (C) Expression of p-ERK1/2 and ERK1/2 on day 7 after treatment with A3D8. (D) Ratio of p-ERK1/2 to ERK1/2 protein on day 7 after treatment with A3D8. Data were presented as mean ± standard errors of the mean (n=3). *P<0.05 and **P<0.01. CD, cluster of differentiation; hAMSCs, human amniotic mesenchymal stem cells; p-, phosphorylated; t-, total; NC, negative control group; PG, positive drug group.