Table I.
Gene | Primer sequence (5′→3′) | Length of product (bp) |
---|---|---|
Col2a1 | F: CAACACTGCCAACGTCCAGAT | 121 |
R: TCTTGCAGTGGGCTGCCTTAT | ||
Sox9 | F: GCGGAGGAAGTCCGTGAAGA | 82 |
R: GAAGATGGCGTTGGGGGAGA | ||
Acan | F: GTGCCTATCAGGACAAGGTCT | 167 |
R: GATGCCTTTCACGACGACTTC | ||
CD44 | F: CTGCCGCTTTGCAGGTGTA | 109 |
R: CATTGTGGGCAAGGTGCTATT | ||
ERK1 | F: CTACACGCAGTTGCAGTACAT | 157 |
R: CAGCAGGATCTGGATCTCCC | ||
ERK2 | F: TCTGGAGCAGTATTACGACCC | 134 |
R: CTGGCTGGAATCTAGCAGTCT |
F, forward; R, reverse; Col2a1, collagen type II α 1 chain; Sox9, SRY-box transcription factor 9; Acan, aggrecan.