Function of the mitotic Ran GPS in spindle assembly. (A) In prometaphase, major centrosomal and non-centrosomal microtubule-organizing centers are located close to mitotic chromosomes and are exposed to high RanGTP concentrations, strongly promoting the local nucleation and stabilization of microtubules that is induced by RanGTP- and importin-regulated SAFs. The SAFs bind to and become concentrated on the nascent microtubules and promote their reorganization into bipolar spindles. During spindle bipolarization, distribution of some SAFs is biased towards specific sites on spindle microtubules. The two examples shown are TPX2, which concentrates at the spindle poles, and HURP, which relocates to kinetochore fibers close to the mitotic chromosomes in bipolar spindles. RanGTP- and exportin-1-regulated mitotic cargos are recruited by cargo-specific mechanisms to centromeres (survivin), kinetochores (RanBP2-RanGAP-SUMO) and centrosomes (NPM1) – either alone (survivin) or colocalizing with exportin 1 (RanBP2-RanGAP-SUMO and NPM1). (B) Switch-like induction of mitotic spindle assembly by multiple SAFs that are incrementally activated by the RanGTP gradient. (Left) SAFs that are inhibited by binding to importins in the cytoplasm are partially activated around the chromatin by the RanGTP-gradient-induced disassembly of SAF-importin complexes (red, orange and yellow lines). As a result, multiple Ran-GPS-directed overlapping gradients of active SAFs surround the mitotic chromosomes and cooperatively promote spindle assembly as described in A. There is only partial inhibition of SAFs in the cytoplasm, but this is balanced by the activity of microtubule depolymerizers (blue lines). Some microtubule depolymerizers, such as stathamin/Op18, are inhibited by chromatin signals, further promoting the localized polymerization of microtubules. (Right) RanGTP-induced local incremental activation of SAFs, in combination with microtubule destabilizers, produces a robust switch-like activation of the mitotic spindle assembly around chromatin.