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. 2020 Apr 23;13:3449–3466. doi: 10.2147/OTT.S248507

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© 2020 Kong et al.

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Knockdown of ELF3 inhibits cell proliferation, migration, and invasion of pancreatic cancer cells. (A) The transfection efficiency of three different siRNAs targeting ELF3 in AsPC-1 and PANC-1 cell lines detected by Western blotting. (B) CCK-8 assay was employed to detect the effect of knockdown of ELF3 in the proliferation ability of pancreatic cancer cells. (C) Representative images of colony formation assay from control, sh-control, and sh-ELF3 group. (D) Statistical analysis of the results from colony formation assay. (E) Representative images of wound-healing assay from control, sh-control, and sh-ELF3 group of AsPC-1 and PANC-1 cells. (F) Statistical analysis of the results from wound-healing assay. (G) Representative images of transwell assay from control, sh-control and sh-ELF3 group of AsPC-1 and PANC-1 cells. (H) Statistical analysis of the results from transwell assay. Data are presented as means ± SEM (n = 3 per group). *p < 0.05, **p < 0.01 vs the sh-control group.