Figure 6.

Suppression of ELF3 is required for the miR-1224-5p-mediated inhibition of proliferative, migrative and invasive abilities of pancreatic cancer cell lines. (A) Cellular proliferation ability was significantly increased after restoration of ELF3 expression by co-transfection gv144-ELF3 plasmid compared with miR-1224-5p mimics group. (B–C) Migratory ability was detected by wound-healing assay after co-transfection of miR-1224-5p mimics and ELF3 overexpression plasmid. (D–E) Invasive ability significantly increased after co-transfection of ELF3 overexpression plasmid compared with miR-1224-5p mimics group. Representative images of transwell assay were provided. (F) CCK-8 assay was used to detect proliferation ability in PANC-1 cell line after co-transfection of miR-1224-5p inhibitor and sh-ELF3. (G–H) Representative images and statistical analysis results of wound-healing assay for different groups. (I–J) Quantification of cell invasion of different groups with different treatments. Representative images and statistical analysis results were provided. Data are presented as means ± SEM (n = 3 per group). *p < 0.05, **p < 0.01 vs the miR-1224-mimics/miR-1224-inhibitor group.