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Flow cytometry. CHO/dPDPN cells were treated with P38B (blue line; 10 or 1 μg/mL), P38B-DM1 (red line; 10 or 1 μg/mL), and normal canine IgG (black line; 10 μg/mL), followed by treatment with fluorescein isothiocyanate-conjugated anti-canine IgG. Fluorescence data were collected using a cell analyzer. IgG, immunoglobulin G; CHO, Chinese hamster ovary; PDPN, dog podoplanin.
