Table 3.
Peptide ion validation data from CID MS/MS mass spectrometry which corresponds with Table 2.
| Spot no. | Protein name | Accession number | Verification CID ions | Peptide position in protein sequence: start–end | m/z | Ions score | Amino acid sequence | Variable modification: carbymidomethyl cysteine (C), oxidation methionine (M) |
|---|---|---|---|---|---|---|---|---|
| 1 | Transthyretin, Chain B | 55669576 | 4 | 12–24 | 1366.74 | 89 | R.GSPAINVAVHVFR.K | |
| 12–25 | 1494.84 | 75 | R.GSPAINVAVHVFRK.A | |||||
| 25–38 | 1522.70 | 88 | R.KAADDTWEPFASGK.T | |||||
| 26–38 | 1394.60 | 47 | K.AADDTWEPFASGK.T | |||||
| 2 | Serum albumin | 23307793 | 5 | 299–310 | 1546.80 | 25 | K.LKECCEKPLLEK.S | 2C |
| 361–372 | 1467.85 | 51 | R.RHPDYSVVLLLR.L | |||||
| 362–372 | 1311.76 | 18 | R.HPDYSVVLLLR.L | |||||
| 438–452 | 1639.94 | 97 | K.KVPQVSTPTLVEVSR.N | |||||
| 439–452 | 1511.85 | 62 | K.VPQVSTPTLVEVSR.N | |||||
| 3 | Serum albumin | 23307793 | 6 | 35–44 | 1226.60 | 57 | R.FKDLGEENFK.A | |
| 123–138 | 1996.92 | 57 | R.NECFLQHKDDNPNLPR.L | C | ||||
| 162–168 | 927.49 | 47 | K.YLYEIAR.R | |||||
| 361–372 | 1467.83 | 57 | R.RHPDYSVVLLLR.L | |||||
| 427–434 | 960.56 | 44 | K.FQNALLVR.Y | |||||
| 438–452 | 1639.92 | 13 | K.KVPQVSTPTLVEVSR.N | |||||
| 4 | Serum albumin | 62113341 | 2 | 570–581 | 1342.63 | 79 | K.AVMDDFAAFVEK.C | |
| 570–581 | 1358.62 | 79 | K.AVMDDFAAFVEK.C | M | ||||
| 5 | Serum albumin | 28592 | 4 | 162–168 | 927.51 | 19 | K.YLYEIAR.R | |
| 361–372 | 1467.84 | 19 | R.RHPDYSVVLLLR.L | |||||
| 427–434 | 960.58 | 33 | K.FQNALLVR.Y | |||||
| 438–452 | 1639.94 | 54 | K.KVPQVSTPTLVEVSR.N | |||||
| 6 | Serum albumin | 62113341 | 5 | 162–168 | 927.49 | 39 | K.YLYEIAR.R | |
| 162–169 | 1083.59 | 19 | K.YLYEIARR.H | |||||
| 234–242 | 1019.58 | 37 | R.AFKAWAVAR.L | |||||
| 427–434 | 960.56 | 45 | K.FQNALLVR.Y | |||||
| 438–452 | 1639.93 | 76 | K.KVPQVSTPTLVEVSR.N | |||||
| 9 | ALB protein | 27692693 | 4 | 107–118 | 1546.79 | 37 | K.LKECCEKPLLEK.S | 2C |
| 169–180 | 1467.84 | 32 | R.RHPDYSVVLLLR.L | |||||
| 235–242 | 960.57 | 39 | K.FQNALLVR.Y | |||||
| 246–260 | 1639.95 | 82 | K.KVPQVSTPTLVEVSR.N | |||||
| 10 | Serum albumin | 62113341 | 4 | 162–168 | 927.51 | 42 | K.YLYEIAR.R | |
| 162–169 | 1083.61 | 18 | K.YLYEIARR.H | |||||
| 234–242 | 1019.6 | 14 | R.AFKAWAVAR.L | |||||
| 427–434 | 960.58 | 26 | K.FQNALLVR.Y | |||||
| 16 | Albumin precursor PRO2619 | 11493459 | 3 | 399–414 | 1910.94 | 41 | R.RPCFSALEVDETYVPK.E | C |
| 440–448 | 1000.60 | 41 | K.QTALVELVK.H | |||||
| 460–471 | 1358.62 | 90 | K.AVMDDFAAFVEK.C | M | ||||
| 28 | ALB protein | 27692693 | 4 | 169–180 | 1467.82 | 54 | R.RHPDYSVVLLLR.L | |
| 235–242 | 960.55 | 49 | K.FQNALLVR.Y | |||||
| 246–260 | 1639.92 | 93 | K.KVPQVSTPTLVEVSR.N | |||||
| 247–260 | 1511.83 | 53 | K.VPQVSTPTLVEVSR.N | |||||
| 81 | No ID | – | – | |||||
| 86 | Transferrin chain A | 49258810 | 2 | 42–50 | 1125.61 | 4 | K.KASYLDCIR.A | C |
| 43–50 | 997.52 | 23 | K.ASYLDCIR.A | C | ||||
| 11 | Apolipoprotein A-I, Chain C, | 90108666 | 3 | 24–40 | 1815.86 | 48 | K.DSGRDYVSQFEGSALGK.Q | |
| 119–131 | 1467.81 | 22 | K.VEPLRAELQEGAR.Q | |||||
| 161–171 | 1301.66 | 28 | R.THLAPYSDELR.Q | |||||
| 13 | Serum albumin | 62113341 | 3 | 162–168 | 927.51 | 25 | K.YLYEIAR.R | |
| 162–169 | 1083.61 | 9 | K.YLYEIARR.H | |||||
| 427–434 | 960.58 | 22 | K.FQNALLVR.Y | |||||
| 14 | No ID | – | – | |||||
| 23 | Albumin precursor PRO2619 | 11493459 | 4 | 251–262 | 1467.85 | 23 | R.RHPDYSVVLLLR.L | |
| 317–324 | 960.58 | 30 | K.FQNALLVR.Y | |||||
| 328–342 | 1639.94 | 69 | K.KVPQVSTPTLVEVSR.N | |||||
| 399–414 | 1910.94 | 7 | R.RPCFSALEVDETYVPK.E | C | ||||
| 25 | Apolipoprotein C-III precursor | 4557323 | 1 | 45–60 | 1716.84 | 79 | K.DALSSVQESQVAQQAR.G | |
| 29 | Albumin precursor PRO2619 | 11493459 | 5 | 251–262 | 1467.84 | 20 | R.RHPDYSVVLLLR.L | |
| 317–324 | 960.57 | 26 | K.FQNALLVR.Y | |||||
| 328–342 | 1639.94 | 64 | K.KVPQVSTPTLVEVSR.N | |||||
| 329–342 | 1511.85 | 7 | K.VPQVSTPTLVEVSR.N | |||||
| 399–414 | 1910.94 | 3 | R.RPCFSALEVDETYVPK.E | C | ||||
| 31 | Albumin precursor PRO1851 | 7770149 | 3 | 468–489 | 2415.14 | 73 | R.QGPVNLLSDPEQGVEVTGQYER.E | |
| 468–491 | 2672.28 | 50 | R.QGPVNLLSDPEQGVEVTGQYEREK.A | |||||
| 610–622 | 1454.73 | 5 | R.TLRVQGNDHSATR.E | |||||
| 36 | ALB Protein | 27692693 | 2 | 235–242 | 960.58 | 28 | K.FQNALLVR.Y | |
| 246–260 | 1639.97 | 67 | K.KVPQVSTPTLVEVSR.N | |||||
| 37 | No ID | – | – | |||||
| 42 | Alpha-1 antitrypsin | 28637 | 4 | 75–84 | 1275.71 | 7 | K.GKWERPFEVK.D | |
| 106–116 | 1403.72 | 12 | K.RLGMFNIQHCK.K | C | ||||
| 107–116 | 1247.61 | 41 | R.LGMFNIQHCK.K | C | ||||
| 158–165 | 1078.55 | 26 | K.FLENEDRR.S | |||||
| 49 | Properdin, B-factor | 57209925 | 3 | 51–74 | 2815.37 | 33 | R.LLQEGQALEYVCPSGFYPYPVQTR.T | C |
| 177–193 | 2056.94 | 28 | K.VGSQYRLEDSVTYHCSR.G | C | ||||
| 183–193 | 1366.61 | 66 | R.LEDSVTYHCSR.G | C | ||||
| 53 | Apolipoprotein A-II | 119573006 | 1 | 48–57 | 1156.68 | 38 | K.SKEQLTPLIK.K | |
| 56 | No ID | – | – | |||||
| 57 | Apolipoprotein A-I, Chain A | 90108664 | 1 | 1–10 | 1226.56 | 27 | DEPPQSPWDR.V | |
| 65 | Albumin | 119626069 | 1 | 66–75 | 1149.65 | 20 | K.LVNEVTEFAK.T | |
| 73 | Alpha-1 antitrypsin, chain | 1942629 | 2 | 11–25 | 1779.75 | 60 | K.TDTSHHDQDHPTFNK.I | |
| 156–168 | 1479.73 | 95 | K.QINDYVEKGTQGK.I | |||||
| 99 | Alpha-1 antitrypsin, Chain | 1942953 | 1 | 11–25 | 1779.77 | 24 | K.TDTSHHDQDHPTFNK.I | |
| 104 | Serum albumin | 62113341 | 3 | 66–75 | 1149.63 | 33 | K.LVNEVTEFAK.T | |
| 162–169 | 1083.61 | 8 | K.YLYEIARR.H | |||||
| 438–452 | 1639.97 | 23 | K.KVPQVSTPTLVEVSR.N | |||||
| 344 | No ID | – | – |
Peptide position in the matched protein, observed mass (m/z) and amino acid sequence are listed for each protein match found.